Multipe Sequence Alignments:
Using the T-Coffee Web Server

  1. Use the html colored output. Red means highly consistent with the library

  2. Check the colored output.

  3. Check the file named template_file

  4. Use the colored output of M-Coffee to quantify the agreement across methods

  5. The pairwise sequence methods are not very consistent. Avoid using them with Expresso if you have enough structural template (i.e. one template for each sequence in the dataset)

Questions should be sent to C.Notredame